ISOLUTE® PPT+
Protein Precipitation Plates
Ordering Information
| Part Number |
Description |
Quantity |
| 120-2040-P01 |
ISOLUTE PPT+ fixed well plate, 2 mL |
1 |
| 120-2040-R |
ISOLUTE Array PPT+ wells, 1 mL |
100 |
| 120-2040-T |
ISOLUTE Array PPT+ wells, 2 mL |
100 |
| 120-1000-P01 |
ISOLUTE Array base plate |
1 |
| 120-1200 |
Strip of 8 base plate sealing plugs* |
50 |
| 120-1201 |
Luer adaptors (to fit any standard sample processing manifold) |
25 |
| 120-1202 |
Well removing tool |
1 | *Required when processing a partially populated ISOLUTE Array PPT+ plate.
Benefits
• 'Solvent first' methodology
• Eliminate cloudy extracts
• No well blockage
• No vortex mixing required
• Optimized for automation
'Solvent First' Procedure Maximizes Precipitation Efficiency
The proprietary functionalized bottom frit holds up organic precipitation solvent while the plasma sample is added, maximizing contact time and giving the most efficient precipitation effect. No vortex mixing is required. The filtrate does not pass into the collection plate until vacuum is applied.
Depth Filter Eliminates Well Blocking
Unlike membrane based devices, the system has an optimized porosity distribution and acts as a depth filter, holding up the precipitated protein without well blockage.
Rapid Non-Selective Sample Clean-up
Using the 'solvent first' methodology, ISOLUTE PPT+ plates consistently produce clear filtrates. This reduces ion suppression and improves the accuracy of LC-MS/MS analysis compared to alternative protein precipitation procedures. Up to 96 samples containing acidic, basic or neutral drug compounds can be purified simultaneously in less than ten minutes.
Technical Details
'Solvent First' Methodology using ISOLUTE PPT+ Protein Precipitation Plates
• ISOLUTE PPT+ plates can be used to process plasma sample volumes from 15 to 400 µL. Serum or blood samples can also be processed.
• The recommended crash solvent is acetonitrile, at a solvent: plasma ratio of 3:1 (v/v). Acetonitrile has been found to be a more effective solvent for precipitating protein than methanol; it produces filtrates with a lower protein concentration.
• Alternatively, acid precipitation may be used e.g., 1 M trichloroacetic acid (TCA) in a 3:1 (v/v) ratio (TCA / plasma). The same dispensing method should be used for these alternative precipitating solvents, and no dripping will occur until vacuum is applied.
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