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Home > Product > 파티클 > Micromod > Silica
제 품 명 : sicastar® -M 자성입자
silica particle
제 조 사 : micromod
제품코드 : 39-00-153
 
   
상세설명


sicastar® -M 자성입자
silica particles

Magnetic silica particles :
 
  • sicastar® -M
    		•  magnetic silica particles
     
  • sicastar® -M -CT
    		•  cluster-typed magnetic silica particles
     
  • sicastar® -M -CT -greenF and redF
    		•  magnetic fluorescent silica particles
     
  • nanomag® -silica
    		•  silica-fortified magnetic dextran nanoparticles

    Magnetic silica particles (sicastar® -M)
    • produced by hydrolysis of orthosilicates in the presence of magnetite
    • have a hydrophilic surface with terminal Si-OH-bonds
    • have monomodal size distributions with mean diameters of 350 nm or 1.5 µm
    • can easily be separated with conventional permanent magnets
    • extremely stable in organic solvents and at high temperatures
    • designed with the surface functionalities OH (plain), NH2, COOH, SH, N-hydroxysuccinimide (NHS) and epoxy for the covalent binding of proteins, antibodies or other molecules
    • available with covalently bound proteins (avidin, streptavidin, protein A, albumin) and biotin
    • can be offered with the nickel(II) chelator nitrilotriacetic acid (NTA) or ready to use with the corresponding nickel complex (Ni-NTA) for the binding of histidine labeled proteins
    • available with a hydrophobic octadecyl (C18) surface
    • deliverable with an organic polymer shell (core-shell method) on request

    Product
    Name    		 sicastar® -M
    Surface plain, NH2, COOH, epoxy, NTA, NHS
    avidin, streptavidin, proteinA, albumin(BSA), biotin
    Ni-NTA, C18, SH
    Size 1.5 μm, 350 nm(39-00-352)
    Solid
    content    		 10, 25, 50 mg/ml
    Quantity 2, 5, 10 ml, 0.5g(39-15-153)
    Composition magnetic silica particles
    Polydispersity
    Index
    < 0.2 39-00-352
    n/a the rest
    Shape spherical
    Density 2.5 g/㎤
    Magnetization 6.0 emu/g particles (H =1000 Oe)
    Saturation
    Magnetization    		 > 8.0 emu/g particles (H > 10.000 Oe)
    Stable in aqueous buffers pH > 3, organic solvents
    Not
    stable in    		 hydrofluoric acid, strong basic media, e.g. 6 M NaOH
    Product
    Form
    aqueous suspension 39-00-153, 39-00-352, 39-01-153, 39-02-153
    39-08-153, 39-11-153, 39-26-153, 39-48-153
    suspension in PBS buffer (pH = 7.4),
    stabilized in 0.02 % sodium azide    		 39-18-153, 39-19-153, 39-20-153, 39-21-153
    suspension in 50 % (v/v) aqueous ethanol 39-51-153
    dried microspheres 39-15-153
    Surface Charge
    Density
    1 μmol/g 39-01-153, 39-02-153
    5.2 μmol/g 39-00-153
    1.4 - 1.6 μg streptavidin / mg particle 39-19-153
    1.4 - 1.6 μg protein A / mg particle 39-20-153
    1.4 - 1.6 μg albumin(BSA) / mg particle 39-21-153
    n/a the rest
    Protein Binding
    Capacity
    1.4 - 1.6 μg albumin(BSA) / mg particle 39-02-153
    1.4 - 1.6 μg avidin / mg particle 39-18-153
    n/a the rest
    Additional
    Remarks
    Storage at 2 - 8°C 39-08-153, 39-15-153, 39-18-153
    39-19-153, 39-20-153, 39-21-153
    Storage at room temperature the rest
    TECHNOTES
    39-00-153 Isolation of plasmid-DNA: 5-10 μg (from 1-1.5 ml overnight culture),
    OD (260 nm)/ OD (280 nm) > 1.9

    Product
    code    		 Surface Product
    From    		 Size Solid content
    (mg/mL)    		 Quantity
    (mL)
    39-00-153 plain aqueous suspension 1.5 µm 50 10
    39-00-352 350 nm 25 10
    39-01-153 NH2 1.5 µm 50 10
    39-02-153 COOH 1.5 µm 50 10
    39-08-153 epoxy 1.5 µm 50 10
    39-11-153 NTA 1.5 µm 50 10
    39-15-153 NHS dried microspheres 1.5 µm n/a 0.5 g
    39-18-153 avidin suspension in PBS buffer (pH = 7.4),
    stabilized in 0.02 % sodium azide    		 1.5 µm 10 2
    39-19-153 streptavidin 1.5 µm 10 2
    39-20-153 proteinA 1.5 µm 10 2
    39-21-153 albumin(BSA) 1.5 µm 10 5
    39-26-153 biotin aqueous suspension 1.5 µm 25 5
    39-48-153 Ni-NTA 1.5 µm 25 10
    39-51-153 C18 suspension in 50 % (v/v) aqueous ethanol 1.5 µm 25 10
    39-93-153 SH aqueous suspension 1.5 µm 50 10
     

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